Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs

The current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in Escherichia coli isolated from canine diarrheal cases. Fecal samples were collected 77 dogs with clinical diarrhea 10 non-diarrheic dogs. Breed, age sex, manifestations recorded. Samples plated on MacConkey EMB agars, followed by standard isolation procedures identification E. coli. An susceptibility test using disc diffusion method performed against Ampicillin, Tetracycline, Trimethoprim, Gentamicin, Streptomycin. PCR used if isolates carry intimin adherence protein (eaeA) genes. Body temperature, respiratory, heart rates significantly higher than detected eaeA gene 44(69.8%) 63 diarrheic All resistant ampicillin, 55(87.3%), 47(74.6%), 29(46.0%), 19(30.2%) these tetracycline, trimethoprim, gentamicin, streptomycin, respectively. frequency 81.0, 52.4, 41.3, 33.3, 23.8 9.5% for CITM, tet (B), dfrA1, aac (3)-IV, aadA1 (A), Overall, 6(9.4%), 16(25.4%), 41(65.1%) positive one, two, three, or four genes, In conclusion, high (69.8%) 9.5-81.0% could be responsible episodes, which may have posed therapeutic implications affected